http://www.FreeScienceLectures.comWe travel inside nucleus to see how the DNA replicates.When DNA replicates its strands are separated by enzine helicase.Single-stranded DNA binding proteines keep the strands from (...?).One DNA strand encodes the leading strand using DNA Polymerase III.Just watch to see what is going on.---It's Never too Late to Study: http://www.FreeScienceLectures com---Notice: This video is copyright by its respectful owners.The website address on the video does not mean anything.---
It is a very excellent animation which explains the hiv replication very clearly.For free download of this video please visit my webpage http://rufusrajadurai.wetpaint.com/And other 3D animation videos visit http://rufusrajadurai.wetpaint com/page/3D+Medical+Animation+LibraryRegards,Dr.RufusThe Lyrics of this video is hereTargeting HIV replicationThe replication of HIV 1 is a multi-stage process.Each step is crucial to successful replication and is therefore a potential target of antiretroviral drugs.Step one is the infection of a suitable host-cell, such as a CD4-positive T-lymphocyte.Entry of HIV into the cell requires the presence of certain receptors on the cell surface, CD4 -- receptors and co-receptors such as CCR5 or CXCR4.These receptors interact with protein-complexes, which are embedded in the viral envelope.These complexes are composed of two glycoproteins:an extracellular gp 120 anda transmembrane gp 41When HIV approaches the target cell gp120 binds to the CD4-receptors. This process is termed attachment.It promotes further binding to a co-receptor. Co-receptor binding results in a conformational change in gp120.This allows gp41 to unfold and insert its hydrophobic terminus into the cell membrane.Gp 41 then folds back on itself.This draws the virus towards the cell and facilitates the fusion of their membranes.The viral nucleocapsid enters the host cell and breaks open releasing two viral RNA-strands and 3 essential replication enzymes:Integrase, Protease and Reverse Transcriptase.Reverse Transcriptase begins the reverse transcription of viral RNA.It has two catalytic domains:The Ribonuclease-H active siteAnd the polymerase active siteHere single stranded viral RNA is transcribed into an RNA-DNA double helix. Ribonuclease- H breaks down the RNA.The polymerase then completes the remaining DNA-strand to form a DNA -- double helix.Now Integrase goes into action.It cleaves a dinucleotide from each 3-prime end of the DNA creating two sticky ends.Integrase then transfers the DNA into the cell nucleus and facilitates its integration into the host cell genome.The host cell genome now contains the genetic information of HIV.Activation of the cell induces transcription of proviral DNA into messenger RNA.The viral messenger RNA migrates into the cytoplasm where building blocks for a new virus are synthesised.Some of them have to be processed by the viral protease.Protease cleaves longer proteins into smaller core proteins.This step is crucial to create an infectious virus.Two viral RNA-strands and the replication enzymes then come together and core proteins assemble around them forming the capsid.This immature particle leaves the cell acquiring a new envelope of host and viral proteins.The virus matures and becomes ready to infect other cells.HIV replicates billions of times per day destroying the hosts` immune cells and eventually causing disease progression.Drugs which interfere with the key steps of viral replication can stop this fatal process.Entry into the host cell can be blocked by fusion inhibitors for example.Inhibition of reverse transcriptase by nucleoside inhibitors or by non-nucleoside Reverse Transcriptase- inhibitors is part of standard antiretroviral regimens.The action of Integrase can be blocked.Protease inhibitors are also part of standard antiretroviral therapy.Each blocked step in viral replication is a step towards better control of HIV disease.Script, Storyboard, Art Direction by: Frank Schauder, MDAnimation: MACKEVISIONPublicity: Dr.Rufus Rajadurai.MD.,D.DENS.,
Biology Project(Just in case you didn't know... We worked on this at like 4am in the morning... yes..yes... LAST MINUTE project!! but turned out really well!!)Lyrics:Deox / y-ribo / nuclei / ic-acid / is made from two strands, a double helixSo then, the strands come apart, Nitrogen bases, they startto unzip By DNA helicaseH2 bondsBetween adenine and thymineH2 bonds Between cytosine and guanineH2 bondsThe ladder structure is strongest inPhosphodiester linksD-N / A Pol-ym / er-ase Builds the new strandFrom the / 5" to 3"DirectionH2 bondsBetween adenine and thymineH2 bonds Between cytosine and guanineH2 bondsThe ladder structure is strongest inPhosphodiester linksNow I can see that its replicatingIn a way thats quite interesting (YEAH!)No matter the distance, the leading strandIs followed by the lagging oneFinally...the ligase Seals up all the breaksA new....a new....a new....DNNNNNNNNNNA!And it starts again!!Oh, how I love bi-olo-gy (yeah yeah yeah)It's better than chemistryAnd next time I have to go pee (I know)what's happening inside of me
Molecular BiologyThis movie show the cell wrapping and DNA replication filmed or computermade. Biologia molecular: Esse filme mostra a divisão celular filmada e a replicação do DNA por computação gráfica.
Detailing the Bulk CD & Bulk DVD Replication process. Follows Bulk Manufacturing from the glass mastering (as opposed to Duplication DVDR) to pressing the final DVD and CDs then the DVD and CD printing. Finally testing the finished CDS and DVDS. Also details how Music CDS are pressed & duplicated. Enjoy! Visit www.dvdreplication.co.nz for more details.
This is my replication of the "Imhotep Radiant Oscillator". It works. Thanks go to the many people envolved in this project. This device runs on 12 volts and lights a 110 volt fluorescent bulb--- WHILE IT IS CHARGING ANOTHER 12 volt BATTERY!! Amazing.
http://www.replicationasia.com What's the difference betweenDuplication and Replication?Another helpful video from Replication Asia that demystifies the bulk DVD and CD manufacturing process. From the terms used in the industry thru to final packaging options.
http://www.FreeScienceLectures.comHIV replication is a complex multi-staged process that includes crucial steps taking place on the exterior as well as the interior of the target host cell.The first three steps involved in cell entry are termed attachment, co-receptor binding and fusion. Each step is crucial to successful viral reproduction.Once HIV virus penetrates the cell, it releases its RNA into the cell.---It's Never too Late to Study: http://www.FreeScienceLectures com---Notice: This video is copyright by its respectful owners.The website address on the video does not mean anything.---
